Bench Calculators

1. 01C1V1 = C2V2 solved for whichever of the four quantities is left empty
2. 02Molar, mass per volume, fold and percent concentrations, each quantity in its own unit
3. 03The diluent volume to add and the fold dilution, beside the solved quantity
4. 04A refusal, not a negative volume, when the asked-for dilution would raise the concentration
5. 05Master mix from the vendor's own published reaction setup, scaled to any reaction volume with every final concentration kept
6. 06Water made up to volume and the template added per tube, so the mix and the tube volumes are both stated
7. 07Overage as a percent of the mix, and a custom recipe in place of the vendor setup

The arithmetic is checked against the vendor's own reaction-setup table: NEB's 20 µl and 50 µl Phusion setups both come out of one scaling. The panel runs on @ports/bench.

Everything runs client-side. Files are parsed in the browser and projects are saved on this machine, so an unpublished sequence is never uploaded.

Why is there no water volume to type in?

Because the vendor writes it as "to volume": water is whatever the other components leave of the reaction, so it is calculated, and the recipe always adds up to the reaction volume.

Can it convert ng/µl to µM?

No. That needs the molar mass of the molecule, so the two concentrations of a dilution must be in the same kind of unit.