Sequencing · experimental
Sequencing Verification
Answer the question a sequencing result is meant to answer: does this clone match the intended construct. A read is aligned to the reference, graded on ungapped identity and coverage, and every mismatch behind the grade is listed so the verdict can be checked rather than trusted.
Five real reads against one M13 reference, plus three whole-plasmid pairs that span the verdict — a clean clone that passes, a wrong-vector near miss, and constructs too far apart to call
What it does
- 01Read to reference alignment with automatic orientation
- 02Grade per read, with ungapped identity and coverage stated
- 03Coverage bar showing matches, mismatches and gaps along the reference
- 04Mismatch list with positions, navigable back to the trace
- 05Confirmed regions annotated onto the plasmid map
- 06Multiple reads against one reference in a single workspace
What it runs on
Alignment and mutation calling run on the cfutils and sangerseqR ports, golden-validated against those libraries. The panel runs on @ports/constructdiff, @ports/verify.
Everything runs client-side. Files are parsed in the browser and projects are saved on this machine, so an unpublished sequence is never uploaded.
Questions
- What does the grade mean?
- It summarises ungapped identity and coverage of the read against the reference. The underlying numbers and every mismatch are shown next to it, so the grade is auditable.
- Can I verify with more than one read?
- Yes. Several reads can be compared against one reference in the same workspace, which is what a two-primer confirmation needs.
Also searched as
sequencing verification tool · verify plasmid sequence online · compare sanger read to reference · clone confirmation software · construct validation sequencing · variant calling from sanger · plasmid sequencing QC · check a clone against its reference · align a Sanger read to a plasmid · confirm insert orientation · did my cloning work