Analyze
BLAST Search
BLAST where the sequence already is. Pick a target from the open construct rather than pasting bases into a form, run it against the database you need, and bring the answer back onto the map as an annotation at document coordinates.
Nucleotide, protein, an annotated plasmid and a headerless raw sequence: reach blastx, blastp and blastn, and every EMBL-EBI service, on real queries
What it does
- 01All five BLAST programs, against 549 databases, read live from the service registry
- 02Every parameter the service advertises, rendered with its own description and defaults
- 03Targets taken from the open document: features, the current selection, ORFs, or the whole sequence, validated before submission
- 04Hit identifiers, organism, coverage, identity, E-value, bit score, frames and strand
- 05The full pairwise alignment for each hit
- 06A hit written back as a real feature at document coordinates, or imported as its own sequence
- 07Exports of the result as TSV, FASTA, XML, the service report, or SVG
- 08Job history that reopens a past run without resubmitting it
What it runs on
The client is the shared EMBL-EBI Job Dispatcher implementation, with parsing asserted against captured real jobs. Nothing about the database list is hardcoded: it is read from the service's own registry. The panel runs on @ports/comparison, @ports/jobs, @ports/networkLayout, @ports/phylo, @ports/queryAnchored, @ports/vecscreen.
Everything runs client-side. Files are parsed in the browser and projects are saved on this machine, so an unpublished sequence is never uploaded.
Questions
- Does my sequence leave the machine?
- Only when you press Search, and the panel says so before you do. The query is submitted to EMBL-EBI, which runs NCBI BLAST+. Everything else in the workbench runs locally.
- How long does a search take?
- It depends on the database: a protein search against Swiss-Prot returns in seconds, while a nucleotide search against the whole of ENA takes around ten minutes. The measured figures are shown next to the choice rather than left as a surprise.
- Can a hit become an annotation?
- Yes. A hit can be written back as a feature at the coordinates of the query region it aligned to, so the identification lands on the map instead of in a report.
Also searched as
BLAST online · blastn blastp blastx · BLAST a plasmid sequence · identify unknown dna sequence · blast search from genbank file · annotate sequence from blast hit · NCBI BLAST alternative · BLAST from a plasmid file · find homologs of a sequence · megablast online · what is this DNA sequence